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Hb Switching in neonatal cultures. Increase of Hb A synthesis in presence of an erythroid potentiating activity (EPA)
Author(s) -
Testa Ugo,
Vainchenker William,
Guerrasio Angelo,
Beuzard Yves,
BretonGorius Janine,
Rosa Jean,
Lusis A. J.,
Golde David
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041100214
Subject(s) - hemoglobin , cell culture , fetal hemoglobin , beta (programming language) , globin , chemistry , biology , biochemistry , microbiology and biotechnology , fetus , genetics , pregnancy , computer science , programming language
The role of EPA (erythroid potentiating activity) on the growth and on the pattern of hemoglobin synthesis in erythroid colonies from human neonates was investigated. Conditioned medium from the Mo cell line was used as a source of EPA. The results have shown that the addition of Mo medium to cultures determined a significant enhancement of the number and size of BFU‐E and an increase of β chain synthesis. The acceleration of hemoglobin switching is not related to an amelioration of the maturation of the erythroid colonies when grown in the presence of Mo medium. The enhancement of Hb A synthesis induced by Mo medium can directly be related to its EPA, which may operate by two different mechanisms: (1) the recruitment of early erythroid progenitors already preprogrammed to synthesize prevalently β chains, or (2) the modulation of β and γ gene activity in cord blood BFU‐E. Some evidence suggests that the first mechanism does operate.