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Growth of human foreskin fibroblasts in a serum‐free, defined medium without platelet‐derived growth factor
Author(s) -
Weinstein R.,
Hoover G. A.,
Majure J.,
van der Spek J.,
Stemerman M. B.,
Maciag T.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041100105
Subject(s) - foreskin , growth factor , fetal bovine serum , medicine , fibroblast , endocrinology , platelet derived growth factor , platelet , in vitro , epidermal growth factor , chemistry , microgram , ascorbic acid , biology , microbiology and biotechnology , cell culture , biochemistry , platelet derived growth factor receptor , genetics , receptor , food science
The hormones which support growth, in vitro, of normal, neonatal human foreskin fibroblasts were determined. Wheresas thrombin and hydrocortisone were major growth stimulants, platelet‐derived growth factor was not. Human foreskin fibroblasts grew in a serum‐free, biochemically defined medium consisting of epidermal growth factor (100 ng/ml), insulin (100 ng/ml), trasferrin (10 μg/ml), thrombin (1 μg/ml), ascorbic acid (10 μg/ml), and hydrocortisone (5 × 10 −5 M) in a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F‐12, supplemented with ovalbumin (1 mg/ml) and trace elements. The growth achieved was comparable to that achieved with 5% fetal bovine serum. Neither platelet‐derived growth factor, fibroblst growth factor, nor somatomedin activity increased proliferation. This serum‐free medium designated Defined Medium F, provides a biochemically defined system for growth and limited subcultivation of human foreskin fibroblasts in vitro.