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Precursor of mast cells fixed in the skin of mice
Author(s) -
Matsuda Hiroshi,
Kitamura Yukihiko,
Sonoda Takashi,
Imori Tatsuo
Publication year - 1981
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041080315
Subject(s) - mast cell , chimera (genetics) , transplantation , microbiology and biotechnology , precursor cell , bone marrow , biology , degranulation , immunology , chemistry , cell , medicine , biochemistry , receptor , gene
The presence and origin of mast‐cell precursors fixed in the skin tissue of mice were investigated. Giant granules of beige (C57BL/6‐ bg j / bg j , Chediak‐Higashi syndrome) mice were used to distinguish different populations of mast cells. Pieces of the skin were grafted from the intact WBB6F 1 (WB × C57BL/6)F 1 −+/+ mice onto the back of the WBB6F 1 −+/+ mice which had been irradiated and injected with bone marrow cells of C57BL/6‐ bg j / bj j mice ( bg j / bg j −+/+ chimeras). Although the number of mast cells in the skin grafts decreased after the transplantation, the mast‐cell precursors circulating in the bloodstream of bg j / bg j −+/+ chimeras ( bg j / bg j type) did not seem to enter into the skin grafts, because most of mast cells were of +/+ type after the recovery of mast‐cell number to pregrafting levels. As a considerable proportion of +/+‐type mast cells was labeled with 3 H‐thymidine, the recovery of mast‐cell number in the grafts was attributed to the proliferation and differentiation of +/+‐type precursor cells fixed in the skin tissue of the donor. On the other hand, the skin of WBB6F 1 ‐ W / W v mice seemed to be depleted of fixed precursors, because most of mast cells were of bg j / bg j type in skin grafted from WBB6F 1 ‐ W / W v mice to bg j / bg j −+/+ chimeras. Since the fixed precursor cells which proliferate and differentiate into mast cells after skin grafting may be transferred to WBB6F 1 ‐ W / W v mice by bone marrow transplantation, such precursor cells seem to have been derived from the bone marrow.