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Cellular regulatory role of leukotriene B 4 : Its effects on cation homeostasis in rabbit neutrophils
Author(s) -
Sha'Afi R. I.,
Naccache P. H.,
Molski T. F. P.,
Borgeat P.,
Goetzl E. J.
Publication year - 1981
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041080314
Subject(s) - homeostasis , microbiology and biotechnology , rabbit (cipher) , leukotriene , chemistry , leukotriene b4 , biology , immunology , inflammation , asthma , statistics , mathematics
We have found that exogenous leukotriene B 4 modifies calcium homeostasis in rabbit neutrophils in a manner essentially analogous to that of the chemotactic peptide f‐Met‐Leu‐Phe. Leukotriene B 4 causes a rapid and dose‐dependent increase in membrane permeability to calcium and a release of calcium from previously unexchangeable intracellular pool(s). The net result of these changes is to transiently elevate the intracellular level of exchangeable calcium. A stereoisomer of leukotriene B 4 with greatly reduced secretory activity toward neutrophils (5S, 12S‐di HETE) is essentially without effect on the rate of 45 Ca uptake at concentrations equal to those that produce near maximal enhancement by leukotriene B 4 . Leukotriene B 4 , in addition to its effects on calcium metablolism, also increases the rate of 22 Na influx into rabbit neutrophils. The relationships between the action of leukotriene B 4 on calcium homeostasis and the neutrophil‐directed activities of arachidonic acid and its lipoxygenase metabolites are discussed.