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The hexose transport system in the human K‐562 chronic myelogenous leukemia‐derived cell
Author(s) -
Dozier J. C.,
Diedrich D. F.,
Turco S. J.
Publication year - 1981
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041080110
Subject(s) - phloretin , phlorizin , hexose , glucose transporter , glycolysis , anaerobic glycolysis , chemistry , biochemistry , kinetics , cell , carbohydrate metabolism , metabolism , biology , endocrinology , enzyme , insulin , physics , quantum mechanics
Kinetics of glucose transport in K‐562 cells was studied using 3‐0‐methylglucose, a nonmetabolizable analog of glucose. A K m of 3.7 mM and V max of 32.0 nmoles/minute/10 6 cells was found for the process. D‐Glucose, phloretin, and phlorizin competitively inhibit the transport of 3‐0‐methylglucose with Ki values of 4.1 mM, 4.1 μM and 225 μM, respectively, whereas L‐glucose did not inhibit transport at all. The results indicate that K‐562 cells, which are known to have erythropoietic characteristics, possess a glucose carrier system similar to the one in adult human erythrocytes. However, the V max data suggest that more copies of the carrier are present in the malignant cell, presumably to support the high rate of anaerobic glycolysis.