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The reversible inability of isoproterenol‐activated parotid gland cells to initiate DNA synthesis in the hypocalcemic thyroparathyroidectomized rat
Author(s) -
Tsang B. K.,
Whitfield J. F.,
Rixon R. H.
Publication year - 1981
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041070106
Subject(s) - endocrinology , medicine , dna synthesis , intraperitoneal injection , parathyroid hormone , body weight , calcium , subcutaneous injection , chemistry , parotid gland , dna , biochemistry , pathology
An intraperitoneal injection of the beta‐adrenergic drug dl‐isoproterenol hydrochloride (100 mg/kg body weight) into male (190–210 g) albino rats caused two cyclic AMP surges (peaking at 10 minutes and again between 8 and 12 hours) and the initiation of DNA synthesis (between 16 and 20 hours) in the parotid glands. The parotid cells in hypocalcemic thyroparathyroidectomized rats still responded to isoproterenol injection by generating the two cyclic AMP surges, but they did not initiate DNA synthesis unless a blood calcium‐elevating combination of parathyroid hormone (50 USP units/100 g of body weight) and 1α,25 (OH) 2 vitamin D 3 (200 pmoles/100 g of body weight) was injected along with the isoproterenol.