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Effects of parathyroid hormone and cyclic AMP analogues on the activity of ornithine decarboxylase and expression of the differentiated phenotype of chondrocytes in culture
Author(s) -
Takigawa M.,
Takano T.,
Suzuki F.
Publication year - 1981
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041060212
Subject(s) - ornithine decarboxylase , medicine , endocrinology , parathyroid hormone , sodium butyrate , cyclic adenosine monophosphate , adenosine , chondrocyte , chemistry , dna synthesis , calcitonin , guanosine , nucleotidase , biology , cell culture , enzyme , in vitro , biochemistry , calcium , genetics , receptor
Parathyroid hormone (PTH) greatly increased the level of adenosine 3′,5′ cyclic monophosphate (cAMP) in rabbit costal chondrocytes in culture 2 minutes after its addition. PTH, as well as N 6 O 2 ' dibutyryl adenosine 3′,5′ cyclic monophosphate (DBcAMP) and 8 Bromo adenosine 3′,5′ cyclic monophosphate (8 Br‐cAMP) induced ornithine decarboxylase (ODC; L‐ornithine carboxylyase; EC 4.1.1.17), which reached a maximum 4 hours after their addition. Neither cAMP, N 6 O 2′ dibutyryl guanosine 3′,5′ cyclic monophosphate (DBcGMP), nor sodium butyrate increased the activity of the enzyme. PTH had no effect on DNA synthesis, while DBcAMP and 8 Br‐cAMP decreased DNA synthesis. Expression of the differentiated phenotype of chondrocytes in culture was also induced by PTH, DBcAMP, and 8 Br‐cAMP, but not by cAMP, DBcGMP, or sodium butyrate, as judged by morphological change. Glycosaminoglycan synthesis, a characteristic of the cartilage phenotype, began to increase 8 hours after addition of PTH or DBcAMP, reaching a plateau 32 hours after their addition. These findings suggest that PTH induces increase of ODC activity and expression of the differentiated phenotype of chondrocytes through increase of cAMP and that induction of ODC is closely related to expression of the differentiated phenotype of chondrocytes.