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Human dermal microvascular endothelial cells in vitro: Effect of cyclic AMP on cellular morphology and proliferation rate
Author(s) -
Davison P. M.,
Karasek M. A.
Publication year - 1981
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041060211
Subject(s) - foreskin , umbilical vein , microvessel , biology , endothelium , endothelial stem cell , in vitro , intracellular , cell culture , cell growth , dermis , human umbilical vein endothelial cell , endocrinology , microbiology and biotechnology , medicine , angiogenesis , anatomy , biochemistry , cancer research , genetics
Macrovascular endothelial cells isolated from the human umbilical vein and microvessel endothelium from the newborn foreskin dermis differ in their requirements for optimal growth in vitor. In the presence of 5 x 10 −4 M dibutyryl cyclic AMP (Bt 2 cAMP), human dermal microvessel endothelial cell proliferation rate increased to give a cell number of 203% of control values by day 10 in culture. The cells retained their characteristic endothelial cell morphology, reached confluence, and could be serially passaged. Cells grown in the absence of Bt 2 cAMP did not proliferate readily and grew in a disorganized pattern. The effect of Bt 2 cAMP on microvascular endothelial cell proliferation rate and morphology could be duplicated by cholera toxin (CT) used together with isobutyl methyl‐xanthine (IMX). These agents were found to elevate intracellular levels of cyclic AMP in microvascular endothelium over 40‐fold. Human umbilical vein cells in culture failed to respond to either Bt 2 cAMP or CT together with IMX. The growth‐promoting effect of dibutyryl cyclic AMP (Bt 2 cAMP) on human foreskin dermal microvascular endothelium in vitro is in marked contrast to the lack of response of human umbilical vein cells. These results provide further evidence of differences in the mechanisms that regulate macro and microvessel endothelial cell proliferation in vitro.