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Biophysical properties of human astrocytic brain tumor cells in cell culture
Author(s) -
Black Peter McL.,
Kornblith Paul L.
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041050321
Subject(s) - brain cell , cell culture , microbiology and biotechnology , human cell , human brain , cell , tumor cells , neuroscience , chemistry , biology , cancer research , biochemistry , genetics
For malignant cells cultured from a human astrocytoma, electrophysiological characteristics of the plasma membrane included specific resistivity of 446.82 ± 279.5 ohm·cm 2 , specific capacitance of 0.758 ± 0.52 microfarads/cm 2 , time constant 0.318± 0.10 msec. The resting membrane potential averaged‐14.07 ± 7.4 mV; the mean input resistance 8.1 ± 4.0 megohms. The average cell area was 1638 ± 585 ± 2 for contactual and 1919 ± 989 ± 2 for noncontactual cells. Changes in input resistance and resting membrane potential were observed with increasing time in culture, possibly reflecting cell cycling. There did not appear to be electrical coupling in this cell line.