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In vitro development of CFU‐E and BFU‐E in cultures of embryonic and post‐embryonic chicken hematopoietic cells
Author(s) -
Samarut J.,
Bouabdelli M.
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041050320
Subject(s) - blastoderm , biology , embryonic stem cell , haematopoiesis , primitive streak , erythropoiesis , yolk sac , microbiology and biotechnology , erythropoietin , embryogenesis , progenitor cell , in vitro , colony forming unit , embryo , andrology , stem cell , gastrulation , endocrinology , biochemistry , medicine , genetics , anemia , bacteria , gene
A culture method is proposed for the in vitro development of chicken erythrocytic progenitors. When grown with avian erythropoietin, Colony Forming Unit Erythrocytic (CFU‐E) and Burst Forming Unit‐Erythrocytic (BFU‐E) give rise respectively to erythrocytic colonies and bursts within 3 and 6 days. BFU‐E development is greatly enhanced by pokeweed‐mitogen‐spleen‐cell‐conditioned medium and requires higher erythropoietin concentrations than for CFU‐E. An antigen specific to immature red cells can be detected on CFU‐E but not on BFU‐E, showing that both progenitors represent distinct entities. BFU‐E and CFU‐E are found in embryonic marrow and yolk sac. In the young blastoderm BFU‐E becomes detectable at the primitive streak stage.