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Defective RNA polymerase II in the G 1 specific temperature sensitive hamster cell mutant TsAF8
Author(s) -
Shales Michael,
Bergsagel John,
Ingles C. James
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041050317
Subject(s) - microbiology and biotechnology , mutant , biology , polymerase , rna polymerase ii , gene , locus (genetics) , hamster , rna polymerase , rna polymerase i , chinese hamster ovary cell , cell culture , rna , rna dependent rna polymerase , gene expression , genetics , promoter
TsAF8 is a temperature‐sensitive (TS) mutant of BHK21 cells that arrests at nonpermissive temperatures in the mid‐G 1 phase of the cell cycle. TsAma R ‐1 is a TS for growth mutant of CHO cells with a Ts‐ and α‐amanitin‐resistant (Ama R ) RNA polymerase II activity. Hybrid TsAma R ‐1 x TsAF8 cell lines were constructed at permissive temperatures. Such hybrid cells did not grow at nonpermissive temperatures; the two TS mutations did not complement. Two different Ama R derivatives of TsAF8 were isolated. Each contained only Ama R polymerase II activity, indicating that this RNA polymerase II gene locus in TsAF8 is functionally hemizygous, as would be expected for a locus in which the recessive TsAF8 mutation had occurred. One of these Ama R isolates of TsAF8 had a partially reverted TS + phenotype. Taken together these results suggest that the TS mutation in TsAF8 is in RNA polymerase II.