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A hemopoietic cell line dependent upon a factor in pokeweed mitogen‐stimulated spleen cell conditioning medium
Author(s) -
Hasthorpe Suzanne
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041050221
Subject(s) - biology , mast cell , haematopoiesis , cell culture , microbiology and biotechnology , cell , population , growth factor , receptor , immunology , biochemistry , stem cell , genetics , medicine , environmental health
A continously growing cell line (FMP1.1) has been isolated, which is dependent upon a factor in PWCM for both growth and survival. FMP1.1 appears to be a mast cell, since IgE receptors are present and their graunles react specifically with a mast cell granule stain. The factor in PWCM may be a glycoprotein and has biochemical properties in common with PWCM factors, which stimulate at least four other lineages of hemopoietic colonyforming cell. This line provides a pure population of cells as a model for studying molecular events of hemopoietic precursor cell growth and differentiation.