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The use of 5‐bromodeoxyuridine and irradiation for the estimation of the myoblast and myocyte content of primary rat heart cell cultures
Author(s) -
Masse M. J. O.,
Harary Isaac
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041050203
Subject(s) - myocyte , bromodeoxyuridine , myosin , biology , population , cell , in vivo , cell culture , microbiology and biotechnology , irradiation , andrology , biochemistry , cell growth , genetics , medicine , physics , environmental health , nuclear physics
A method for killing dividing cells (Puck and Kao, '67) was adapted for the elimination of dividing heart muscle cells (myoblasts) in cultures. We have used this method to demonstrate their presence and to estimate their number as well as the number of nondividing heart muscle cells (myocytes) in the neo‐natal rat heart. Cells were cultivated in BUdR (5‐bromodeoxyuridine) 10 −4 M for 3 days and then irradiated with long UV light. The selective elimination of dividing cells led to a loss of myosin Ca 2+ ‐activated ATPase in the cultures. This indicates the presence of dividing cells which contain myosin. The percent of ATPase left after irradiation was 32% of the control in cultures derived from 1‐day postnatal rats and 48% in cultures from 4‐day postnatal rats. This reflects an in vivo shift of myoblasts to myocytes in the muscle cell population as the rat ages.