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Sugar transport in the LLC‐PK 1 renal epithelial cell line: Similarity to mammalian kidney and the influence of cell density
Author(s) -
Mullin James M.,
Weibel Josef,
Diamond Leila,
Kleinzeller Arnost
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041040311
Subject(s) - similarity (geometry) , kidney , sugar , cell culture , chemistry , line (geometry) , epithelium , structural similarity , biochemistry , cell , microbiology and biotechnology , biology , medicine , endocrinology , computer science , genetics , mathematics , geometry , artificial intelligence , image (mathematics)
Cells of confluent cultures of the established pig renal epithelial line, LLC‐PK 1 , accumulate α‐methyl‐D‐glucoside against a concentration gradient. This transport system is strongly inhibited by phlorizin and 6‐deoxy‐D‐glucose, moderately inhibited by phloretin, and only weakly inhibited by 3‐0‐methyl‐D‐glucose, paralleling the situation in mammalian kidney. The time course for the uptake of α‐methyl‐D‐glucoside and for the carrier‐mediated but passive uptake of 3‐0‐methyl‐D‐glucose are identical to those seen in mammalian kidney. Subconfluent cultures of LLC‐PK 1 cells are unable to accumulate α‐methyl‐D‐glucoside, and their transport of this glucose analog is less sensitive to phlorizin inhibition than is the transport system in confluent cultures. Transmission electron micrographs show that cells from subconfluent cultures lack the microvillous surface seen in cells from confluent cultures. Cell density is thus a factor in the occurrence of structural and functional differentiated properties related to transport in these cells.