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The permeability of the human red cell to deuterium oxide (heavy water)
Author(s) -
Karan Daniel M.,
Macey Robert I.
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041040210
Subject(s) - permeability (electromagnetism) , chemistry , reagent , deuterium , heavy water , chromatography , biophysics , biochemistry , membrane , organic chemistry , physics , quantum mechanics , biology
Using a stopped‐flow device, the osmotic water permeability of human red cells to D 2 O and H 2 O was studied as a function of temperature and under the influence of the sulfhydryl reagent paracholoromercuribenzene sulfonic acid (PCMBS), an inhibitor of water transport. The ratio, permeability (D 2 O)/permeability (H 2 O) at each temperature can be predicted simply by assuming that permeability varies inversely with macroscopic viscosity. When water permeability is inhibited with PCMBS, this dependency on viscosity vanishes; the inhibited permeabilities in D 2 O and H 2 O are indistinguishable.