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Megakaryocytopoiesis in culture: Modulation by cholinergic mechanisms
Author(s) -
Burstein Samuel A.,
Adamson John W.,
Harker Laurence A.
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041030205
Subject(s) - endocrinology , medicine , cholinergic , dna synthesis , forskolin , phosphodiesterase , agonist , megakaryocytopoiesis , sodium nitroprusside , biology , chemistry , haematopoiesis , stimulation , receptor , biochemistry , microbiology and biotechnology , dna , enzyme , stem cell , nitric oxide , megakaryocyte
Treatment of murine bone marrow cultures with the cholinergic agonist carbamylcholine enhanced megakaryocytic colony growth by as much as 65%. In contrast, adrenergic agonists had no such effect. Addition to cultures of dibutyryl cyclic GMP (db‐cGMP) also enhanced megakaryocytic colonies up to 50%, whereas dibutyryl cyclic AMP (db‐cAMP) had no effect. Sodium nitroprus‐side and sodium nitrite, putative guanyl cyclase activators, also enhanced colony numbers, as did imidazole, a postulated cGMP phosphodiesterase inhibitor. Preincubation of marrow for two hours with carbamylcholine resulted in both an increase in colony numbers (58%) and percent of progenitors in DNA synthesis (48%, compared to 14% for controls) as determined by tritiated thymidine suicide studies. Treatment of mice with the acetylcholinesterase inhibitor neostigmine resulted in an increase in CFU‐M/humerus (62%) and percent in DNA synthesis (45%). These data indicate that (1) cholinergic, but not adrenergic, agonists modulate megakaryocytopoiesis in culture; (2) this effect may be mediated by cyclic GMP; and (3) only a brief period of exposure of marrow cells to agonist results in enhancement of megakaryocytic colonies.