Premium
Changes in RNA polymerase II in a cell cycle‐specific temperature‐sensitive mutant of hamster cells
Author(s) -
Rossini Mara,
Baserga Susan,
Huang C. H.,
Ingles C. James,
Baserga Renato
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041030114
Subject(s) - rna polymerase ii , mutant , baby hamster kidney cell , polymerase , microbiology and biotechnology , rna , temperature sensitive mutant , rna polymerase , biology , hamster , cell , chemistry , dna , biochemistry , gene expression , gene , promoter
tsAF8 cells are a temperature‐sensitive mutant of BHK cells that arrest at the nonpermissive temperature in the G 1 phase of the cell cycle. The activity of solubilized RNA polymerase II and its ability to bind [ 3 H]‐γ‐amanitin decrease in tsAF8 cells at 40.6°, with a half‐life of ∼ 10 hr. No appreciable changes occur in these two parameters in tsAF8 cells at 34° or in BHK cells at either 34° or 40.6°. Protein synthesis is not appreciably affected for at least 24 hr after tsAF8 cells are shifted to 40.6°. These results indicate that in tsAF8 cells at the nonpermissive temperature, there is a defect in either the synthesis, the assembly, or the stability of RNA polymerase II, and that the loss of RNA polymerase II molecules is not due to widespread cellular damage.