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The stimulation of the initiation of DNA synthesis by fibroblast growth factor in swiss 3T3 cells: Interactions with hormones during the pre‐replicative phase
Author(s) -
Richmond K. M. Veronica,
Kubler AnneMarie,
Martin Francisco,
De Asua Luis Jimenez
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041030112
Subject(s) - dna synthesis , fibroblast growth factor , stimulation , population , endocrinology , medicine , lag , hormone , prostaglandin , growth factor , fibroblast , biology , dna , chemistry , microbiology and biotechnology , cell culture , biochemistry , genetics , receptor , environmental health , computer science , computer network
Fibroblast Growth Factor (FGF) stimulates quiescent Swiss 3T3 cells to initiate DNA synthesis and divide. Cells begin to enter the S‐phase after a lag of 13–15 hr, and the rate of initiation of DNA synthesis in the population can be quantified by a first order rate constant, k. A subsaturating concentration of FGF may establish the lag phase, while the value of k is dependent on the FGF concentration present during the second half of the lag phase. Insulin and hydrocortisone enhance the effect of FGF by increasing k without changing the lag phase, and they can act when added at any time after FGF. Prostaglandin E 1 (PGE 1 ) causes a decrease in k and a lengthening of the lag phase, and acts only when added during the first 8 hr. None of these agents stimulate DNA synthesis in the absence of FGF. These results show that the stimulation of growth by FGF follows the same basic pattern as was previously shown with Prostaglandin F 2α (PGF 2α ). However, since hydrocortisone inhibits stimulation by PGF 2α when added during the first 4 hr of the lag phase, there are clearly differences in some events stimulated by the two growth factors.