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NAD turnover in microplasmodia of physarum polycephalum
Author(s) -
Manser Tim,
Olivera Baldomero M.,
Haugli Finn B.
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041020312
Subject(s) - physarum polycephalum , nad+ kinase , nicotinamide , biochemistry , physarum , nicotinamide adenine dinucleotide , biology , metabolism , chemistry , enzyme , microbiology and biotechnology
The rate of NAD turnover in microplasmodia of Physarum polycephalum was investigated using a double labeling technique with ( 14 C)‐adenine or adenosine and ( 3 H)‐nicotinamide. The half‐life of an NAD molecule in Physarum was estimated to be 25 min, which is shorter than in either E. coli or human cell lines. The half‐life of NAD in the presence of an inhibitor of NADase and poly ADPR synthase, 5‐methyl‐nicotinamide, was also investigated, but found to be indistinguishable from controls. The possible reasons for this and for the rapid turnover is discussed in the light of the known functions for NAD in prokaryotes and eukaryotes.