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Production of a colony‐stimulating factor following differentiation of leukemic myeloblasts to macrophages
Author(s) -
Maeda Michiyuki,
Ichikawa Yasuo
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041020307
Subject(s) - lipopolysaccharide , acute myeloblastic leukemia , granulocyte macrophage colony stimulating factor , macrophage , cell culture , colony stimulating factor , stimulation , leukemia inhibitory factor , biology , cellular differentiation , myeloid , immunology , granulocyte , leukemia , microbiology and biotechnology , chemistry , cytokine , in vitro , interleukin 6 , haematopoiesis , endocrinology , stem cell , biochemistry , gene , genetics
Leukemic cells in the myeloblastic stage from a murine myeloid leukemia cell line (M1) were induced to differentiate to macrophages by lipopolysaccharide (LPS) from Gram‐negative bacteria. A granulocyte‐macrophage colony‐stimulating factor (CSF) was produced only during differentiation. After induction of differentiation, the continued presence of LPS was necessary to stimulate the macrophages to release CSF. In contrast, a macrophage cell line (Mm‐1) derived from the M1 line produced CSF without LPS‐stimulation, but CSF release was stimulated by the presence of LPS.