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The regulation of haemopoiesis in long‐term bone marrow cultures: I. Role of L‐cell CSF
Author(s) -
Dexter T. M.,
Shadduck R. K.
Publication year - 1980
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041020302
Subject(s) - antiserum , haematopoiesis , granulocyte , bone marrow , stromal cell , colony stimulating factor , in vitro , macrophage , biology , cell culture , granulocyte macrophage colony stimulating factor , cell , immunology , microbiology and biotechnology , stem cell , antibody , biochemistry , cancer research , genetics
The effects of L‐cell conditioned medium which contains granulocyte/macrophage colony stimulating factor (CSF); of highly purified L‐cell CSF; and the antiserum directed against L‐cell CSF, have been investigated in long‐term murine bone marrow cultures. Treatment of cultures with CSF containing conditioned medium led to a rapid decline in haemopoiesis. However, this inhibition of in vitro haemopoiesis is probably caused by materials other than CSF, since the addition of highly purified L‐cell CSF had no appreciable effect upon long‐term haemopoietic cell proliferation or differentiation. Furthermore, the inhibitory activity of L‐cell conditioned medium was not abrogated following neutralization of the CSF activity by CSF antiserum. The direct addition of CSF antiserum did not inhibit granulocyte or macrophage formation. These results suggest that long‐term cultures of murine marrow cells may show extensive interactions with stromal cells which are not influenced by exogenous stimulatory or inhibitory factors.