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Studies on the penetration of mammalian cells by deoxyribonucleoside‐5′‐phosphates
Author(s) -
Waqar M. Anwar,
Taber Robert L.,
Huberman Joel A.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041010207
Subject(s) - hela , deoxyribonucleoside , dna , biochemistry , extracellular , cell , nucleic acid , wi 38 , biology , chemistry , microbiology and biotechnology , ploidy , gene
We have tested the ability of [5′‐ 32 P]‐deoxyribonucleoside monophosphates (dNMPs) to penetrate living mouse fibroblast L cells and human HeLa cells. Under the conditions of our experiments, small numbers of apparently intact dNMP molecules appeared to penetrate into the interior of L cells and be incorporated into DNA. This incorporation was not due to mycoplasma contamination nor to extracellular hydrolysis of the dNMPs followed by resynthesis inside the cell. Under these same conditions, penetration of HeLa cells by intact dNMPs did not occur to a significant extent. However, HeLa cells were capable of hydrolyzing extracellular dNMPs to Pi and deoxyribonucleosides at a much faster rate than L cells. These experiments provide a starting point for attempts to specifically label the DNA in intact, living eukaryotic cells with [ 32 P]‐dNMPs.