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Effect of cytosine arabinoside triphosphate on the incorporation of 3 H‐thymidine in mitochondria isolated from HeLa cells
Author(s) -
Mattoccia L. Pica,
Sorrentino R.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041010104
Subject(s) - cytosine , ethidium bromide , dna , hela , thymidine , mitochondrial dna , mitochondrion , biochemistry , microbiology and biotechnology , in vivo , deoxycytidine , biology , chemistry , cell , genetics , gene , chemotherapy , gemcitabine
HeLa cell mitochondria were allowed to incorporate 3 H‐thymidine in a cell free system and the effect of ethidium bromide, cytosine arabinoside and cytosine arabinoside triphosphate on the labeling of mitochondrial DNA was studied. The labeled products, isolated by sedimentation velocity in CsCl‐ethidium bromide two‐step gradients, showed similar sedimentation profiles as in vivo labeled mtDNA. Cytosine arabinoside triphosphate and ethidium bromide strongly inhibited the labeling of mitochondrial DNA, whereas cytosine arabinoside appeared to be much less effective. Tritiated deoxycytidine was found to be incorporated by isolated mitochondria, whereas cytosine arabinoside was shown to enter the mitochondrial acid‐soluble pool but not to be incorporated in acid‐insoluble form. These results are in agreement with the previously reported findings of in vivo experiments.