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Somatic cell hybrids between totipotent mouse teratocarcinoma and rat hepatoma cells
Author(s) -
Litwack Gerald,
Croce Carlo M.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041010102
Subject(s) - totipotent , teratocarcinoma , somatic cell , microbiology and biotechnology , biology , hybrid , cell , protoplast , somatic fusion , cellular differentiation , genetics , botany , gene
We have produced somatic cell hybrids between totipotent mouse teratocarcinoma cells and rat hepatoma cells. These hybrids were tested for the expression of liver specific functions expressed in the hepatoma cell parent and for their ability to differentiate when injected into nude mice. The results of this study indicate that hybrid cell clones do not resemble either of the parental cells, since they do not produce albumin and tyrosine aminotransferase that are expressed in the rat hepatoma parent, and are incapable of forming either teratocarcinoma or hepatomas when injected in experimental animals.