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Stimulation of chondroitin sulfate synthesis by β‐D‐xyloside in chondrocytes of the proteoglycan deficient mutant nanomelia
Author(s) -
Stearns K.,
Goetinck P. F.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041000104
Subject(s) - chondroitin sulfate , glycosaminoglycan , mutant , chemistry , chondroitin sulfate proteoglycan , proteoglycan , chondroitin , biochemistry , stimulation , sulfate , microbiology and biotechnology , extracellular matrix , biology , gene , organic chemistry , endocrinology
The potential of nanomelic chondrocytes to synthesize chondroitin sulfate was investigated by providing the mutant cells with p‐nitrophenyl‐β‐D‐xyloside, a compound which acts as an artificial acceptor for glycosaminoglycan synthesis. Under these conditions the synthesis of chondroitin sulfate in nanomelic and normal chondrocytes is comparable. The chondroitin sulfate synthesized by the mutant is indistinguishable in molecular size and composition from that synthesized by similarly treated normal chondrocytes.