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Neurotrophic effect of nerve extract on development of tetrodotoxin‐sensitive spike potential in skeletal muscle cells in culture
Author(s) -
Kano Masaakira,
Suzuki Nobuyuki,
Ojima Hisayuki
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040990307
Subject(s) - tetrodotoxin , sodium channel , skeletal muscle , spinal cord , spike potential , microbiology and biotechnology , biology , myocyte , chemistry , embryonic stem cell , sodium , anatomy , neuroscience , biophysics , biochemistry , gene , organic chemistry , depolarization
The effect of the presence of nerve extracts on the development of tetrodotoxin (TTX)‐sensitive sodium channels in cultures of dissociated embryonic chick skeletal muscle cells was examined by measuring the maximum rate of rise of TTX‐sensitive spike potential. The addition of the nerve extract prepared from brain or spinal cord of chick embryos to the culture medium caused an increase in the channel density. Extracts of non‐neural tissues, i.e., lung, kidney, and muscle, were ineffective Liver extract, however, produced an effect similar to the nerve extracts. These resutls suggest that the TTX‐sensitive sodium channels in the muscle cell membrane are regulated by a diffusible chemical substance independently of innervation, and that this substance resides in neural tissues, and perhaps also in liver.