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Production of factors required for cell attachment and spreading is a constitutive property in mouse A9 cells
Author(s) -
Dairkee Shahnaz Hashmi,
Gilbert Michael W.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040990306
Subject(s) - adhesion , substrate (aquarium) , microbiology and biotechnology , cell culture , dilution , cell adhesion , promotion (chess) , cell , suspension culture , biophysics , suspension (topology) , biology , chemistry , biochemistry , genetics , ecology , mathematics , physics , organic chemistry , homotopy , politics , political science , pure mathematics , law , thermodynamics
It is demonstrated here that cells in a suspension culture of an established mammalian cell line release non‐dialyzable factors into their growth medium. These factors are capable of promoting the adhesion and spreading of these cells on a generally non‐attachable substratum and also promote spreading on an adhering substrate. Evidence in presented which demonstrates that the spreading promotion activity of the conditioned medium is dependent on the cell density of the culture from which it was derived. Dilution of the conditioned medium results in a proportionate dilution of the spreading promotion activity. The results clearly demonstrate that the production of this spreading promotion factor is continued even in the absence of cell to substrate attachment.