Premium
Gonadotropin stimulation of cyclic AMP levels in Chinese Hamster Ovary cells in culture
Author(s) -
Evain Daniele,
Anderson Wayne B.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040990116
Subject(s) - chinese hamster ovary cell , intracellular , medicine , stimulation , endocrinology , human chorionic gonadotropin , gonadotropin , follicle stimulating hormone , hormone , extracellular , chemistry , chinese hamster , ovary , biology , in vitro , luteinizing hormone , biochemistry , receptor
When Chinese Hamster Ovary (CHO) cells, incubated in serum‐free medium, are exposed to gonadotropins a transient increase in the intracellular concentration of cyclic AMP is observed. Maximum accumulation of cyclic AMP is noted 30 minutes after addition of either human chorionic gonadotropin (hCG) or follicle stimulating hormone (FSH). Within one to two hours after hormone addition, the intracellular concentrations of cyclic AMP have returned to basal levels. The enhancement of intracellular cyclic AMP levels by hCG is hormone concentration dependent, with maximal stimulation observed at 10 μg/ml hCG. The exogenous addition of gonadotropins also slows the growth rate of CHO cells. This effect on growth seems to be mediated through cyclic AMP since the growth rate of a mutant of CHO cells defective in the catalytic subunit of cyclic AMP dependent protein kinase is only slightly decreased.