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Amino acid transport in normal and Rous sarcoma virus‐transformed chicken embryo fibroblasts
Author(s) -
Nakamura Kenji D.,
Weber Michael J.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040990103
Subject(s) - rous sarcoma virus , amino acid , cycloheximide , embryo , biology , biochemistry , leucine , proline , alanine , phenylalanine , virus , starvation , protein biosynthesis , microbiology and biotechnology , virology , endocrinology , gene
A study was made of the transport of a variety of amino acids by uninfected and Rous sarcoma virus‐infected chicken embryo fibroblasts. Following a period of amino acid starvation, transformed, but not normal cells, showed increased levels of transport for α‐aminoisobutyric acid, proline and alanine, three amino acids which are transported primarily by the A transport system. There was no starvation‐induced increase in the transport of leucine, phenylalanine, lysine, or cycloleucine. In the absence of starvation, normal and transformed cells exhibited comparable rates of amino acid transport. Cycloheximide was able to block the increase in uptake. The enhanced uptake was characterized by an increase in V max for transport and little change in K m . The data demonstrate that an alteration in the regulation of the A amino acid transport system is an early event in malignant transformation by Rous sarcoma virus. However, since this alteration is made manifest only following a period of starvation, our findings suggest that increased amino acid uptake does not play a role in generating the other manifestations of the transformed state seen in cell culture.