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Selective suppression of the transcription of ribosomal genes in mouse‐human hybrid cells
Author(s) -
Perry R. P.,
Kelley D. E.,
Schibler U.,
Huebner K.,
Croce C. M.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980313
Subject(s) - gene , biology , ribosomal rna , genetics , transcription (linguistics) , microbiology and biotechnology , philosophy , linguistics
Mouse‐human hybrid cells that preferentially segregate either mouse or human chromosomes were analyzed for their relative content of mouse and human rRNA genes and for their capacity to transcribe these genes. A distinctive Hind III restriction fragment containing 28S rRNA sequences was used to distinguish between mouse and human rDNA and a set of distinctive loop structures in the 45S pre‐rRNA was used to distinguish between mouse and human gene transcripts. Our results indicate that the genes of only one species are transcriptionally active in these hybrid cells, even though both sets of genes are present.