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Ordered replication of DNA sequences: Synthesis of mouse satellite and adjacent main band sequences
Author(s) -
Dooley Douglas C.,
Ozer Harvey L.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980310
Subject(s) - satellite , satellite dna , replication (statistics) , dna synthesis , dna replication , dna , biology , origin of replication , nuclease , microbiology and biotechnology , computational biology , genetics , gene , physics , genome , virology , astronomy
The replication of mouse satellite DNA was delayed when synchronized 3T3 cells were exposed to low concentrations of hydroxyurea during S phase. It appears that the onset of satellite replication is not a time dependent event, but instead requires that a certain amount of main band DNA be synthesized first. Using hydroxyapatite chromatography and S 1 nuclease digestion, a procedure was developed to quantitate the synthesis of both satellite and neighboring main band sequences. The replication kinetics of satellite determined by this method agree with previous estimates. Main band sequences adjacent to satellite appear to replicate in concert with satellite DNA. The results are discussed and related to the limitations of the techniques utilized.