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Karyology and tumorigenicity of a Simian virus 40‐transformed chinese hamster cell clone
Author(s) -
Lehman John M.,
Trevor Katrina
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980302
Subject(s) - biology , clone (java method) , karyotype , autosome , chinese hamster , microbiology and biotechnology , chromosome , ploidy , virus , staining , population , giemsa stain , virology , g banding , cell culture , genetics , dna , demography , sociology , gene
A pseudodiploid clone of Chinese hamster cells transformed in vitro with Simian virus 40 (SV 40 ) was isolated from soft agar and injected into nude mice through three successive passages with a short in vitro cultivation between each animal inoculation. The original clone and the three subsequent tumor populations were characterized in regard to SV 40 T antigen staining, modal chromosome number, and Giemsa‐banded karyotype. All tumor cell lines maintained the pseudodiploid mode, as well as the positive SV 40 T antigen staining. Nonrandom chromosomal changes included loss of one of the X chromosomes, additions of abnormal variants of chromosomes No. 1 and No. 2, the appearance of unidentified marker chromosomes, and the loss of autosomes No. 5, No. 6, and No. 11. The deletion of one of the X chromosomes occurred with about the same frequency in all cell lines. Additions of abnormal chromosomes No. 1 and No. 2 tended to recur more often in the tumor cell lines than in the original clone. The appearance of marker chromosomes, as well as the loss of autosomes No. 5, No. 6, and No. 11 demonstrated a correlation with tumorigenicity. Yet, the three successive passages of the cells through the animal did not select for a tumor population with a single, homogeneous karyotype.