Premium
Superoxide dismutase specific activities in cultured human diploid cells of various donor ages
Author(s) -
Duncan Michael R.,
Dell'orco Robert T.,
Kirk K. D.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980219
Subject(s) - superoxide dismutase , in vitro , ploidy , fetus , enzyme , andrology , biology , free radical theory of aging , enzyme assay , superoxide , cell culture , cell , microbiology and biotechnology , chemistry , biochemistry , genetics , antioxidant , pregnancy , medicine , gene
It has been postulated that superoxide dismutase (SOD) protects cells from free radical‐induced damage. In these experiments SOD specific activity was measured as established human diploid cell lines from various donor ages progressed through their in vitro lifespans. Significant elevations in activity occurred during the in vitro lifespans of cells from fetal and newborn donors, but no change in activity was detected during the lifespan of cells from an adult donor. In addition, a direct relationship between enzyme activity and donor age was detected with the following relative activities: adult > newborn > fetal. The possible relationship between these findings and the free radical theory of aging is discussed.