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The nature of cells generating human myeloma colonies in vitro
Author(s) -
Hamburger Anne W.,
Kim Mary B.,
Salmon Sydney E.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980213
Subject(s) - in vitro , bioassay , agar , cell culture , myeloma protein , monoclonal antibody , spleen , biology , microbiology and biotechnology , antibody , chemistry , immunology , biochemistry , bacteria , genetics
Freshly explanted human myeloma cells formed colonies of monoclonal plasma cells in soft agar in the presence of medium conditioned by the adherent spleen cells of mineral oil‐primed BALB/c mice. The medium showed peak activity at a dilution of 1:4. 2‐mercaptoethanol or monothioglycerol was necessary for colony formation. Other thiols tested were ineffective in promoting colony growth. Colony‐forming cells adhered to nylon wool, but not glass beads or plastic dishes. The presence of E‐rosetting cells was not required for myeloma colony formation. Antibody prepared against a human myeloma cell line, RPMI 8226, reduced colony formation. These studies demonstrate the usefulness of this bioassay for determining functional properties of the myeloma colony‐forming cell.