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The effect of amino acids on the temperature sensitive phenotype of the mammalian leucyl‐tRNA synthetase mutant ts Hl and its revertants
Author(s) -
Molnar S. J.,
Rauth A. M.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980208
Subject(s) - leucine , isoleucine , valine , amino acid , chinese hamster ovary cell , biochemistry , mutant , transfer rna , biology , amino acyl trna synthetases , enzyme , aminoacyl trna synthetase , protein biosynthesis , extracellular , rna , gene , receptor
The temperature sensitive leucyl‐tRNA synthetase mutant ts Hl and two revertants have been compared to the parental Chinese hamster ovary cells with respect to the effects of amino acid concentrations in the medium on growth. Elevating the leucine concentration 30‐ or 100‐fold allowed ts Hl to grow exponentially at 38.5°C, normally the nonpermissive temperature. Partial revertants that had recovered some enzyme activity required smaller supplements for growth. Measurements of the leucine pools indicated that they respond directly to the extracellular leucine concentration and may mediate the effect. Use of combinations of amino acids confirmed that isoleucine has a similar though weaker effect on ts Hl and identified an even weaker protection by valine. The triple combination of leucine, isoleucine and valine was a much more efficient medium supplement and three times normal concentrations of these amino acids supported growth of ts Hl at 38.5°C. It is postulated that they are acting at their respective aminoacyl‐tRNA synthetases to help stabilize a complex which also contains the mutant leucyl‐tRNA synthetase. The pool size measurements also showed that the leucine pools of ts Hl and a revertant increased 2‐fold more in a response to increased temperature than those of WT. It is suggested that this is a regulatory response to low leucyl‐tRNA synthetase activity and is important in determining growth phenotypes.