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Patterns of peptide synthesis in senescent and presenescent human fibroblasts
Author(s) -
Engelhardt Dean L.,
Lee Gloria TY.,
Moley Jeffrey F.
Publication year - 1979
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040980121
Subject(s) - foreskin , peptide , fibroblast , polyacrylamide gel electrophoresis , cohort , cell , biology , microbiology and biotechnology , chemistry , cell culture , biochemistry , in vitro , medicine , enzyme , genetics
Peptide production in senescent and presenescent human foreskin fibroblasts was measured using 2‐dimensional polyacrylamide gel electrophoresis. This procedure permits the visualization of a cohort of the major peptides being produced. Among this cohort of over 500 peptides only two were found to differ in relative amount in that more was being produced in senescent cells. This difference was confirmed by measurements of the relative intensity of the peptide spot. This difference was senescent cell‐specific and not due to the differences in rate of growth of senescent and non‐senescent cells.