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The effect of oxygen tension on haemopoietic and fibroblast cell proliferation in vitro
Author(s) -
Bradley T. R.,
Hodgson G. S.,
Rosendaal M.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040970327
Subject(s) - oxygen tension , incubation , in vitro , cell culture , fibroblast , progenitor cell , cell , biology , agar , oxygen , cell growth , microbiology and biotechnology , immunology , chemistry , stem cell , biochemistry , genetics , bacteria , organic chemistry
The effects of providing low oxygen tension in the gas phase of two different types of cell culture systems were investigated. The clonal growth of granulocyte‐macrophage progenitor cells in an agar culture system was improved markedly by incubation within a low oxygen tension gas phase (48 mmHg – 6.8%) instead of the conventional air (135 mmHg – 19%), the effects being measured by increases in numbers of colony forming cells detected and in the colony sizes. The increased efficiency of colony formation was observed both with mouse and human marrow cells. A similar effect was observed in a liquid adherence culture system with primary cultures of foetal mouse fibroblasts both at clonal and higher cell densities.