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Polyene macrolide antibiotic cytotoxicity and membrane permeability alterations I. Comparative effects of four classes of polyene macrolides on mammalian cells
Author(s) -
Fisher Paul B.,
Bryson Ver,
Schaffner Carl P.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040970309
Subject(s) - polyene , cytotoxicity , toxicity , membrane permeability , macrolide antibiotics , hamster , chemistry , viability assay , biology , biochemistry , antibiotics , microbiology and biotechnology , membrane , cell , in vitro , erythromycin , organic chemistry
The relationship between polyene macrolide‐induced early membrane damage and cytotoxicity in B1 (hamster), B82 (mouse), and RAG (mouse) cells has been investigated. Filipin (FIL) induced the greatest immediate damage, as monitored by 51 Cr release, followed by mediocidin (MED), amphotericin B‐deoxycholate (Fungizone®) (FZ) and pimaricin (PIM). For long term effect, PIM was the least toxic followed by MED, FZ, and FIL as indicated by 24‐hour survival, 72‐hour viability, and growth rate of cells. In evaluating polyene macrolide‐induced permeability alterations and cytotoxicity two types of interactions with mammalian cells were found: (1) cell toxicity at polyene macrolide levels not eliciting immediate membrane permeability changes; and (2) immediate membrane damage without long range toxicity.