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Hormonal activation of adenylate cyclase in mouse melanoma metastatic variants
Author(s) -
Niles R. M.,
Makarski J. S.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040960311
Subject(s) - adenylate kinase , cyclase , metastatic melanoma , melanoma , hormone , biology , cancer research , medicine , endocrinology , microbiology and biotechnology , chemistry , enzyme , biochemistry , stimulation
The ability of melanocyte stimulating hormone (MSH), adrenocorticotropic hormone (ACTH), and prostaglandin E 1 (PGE 1 ) to stimulate the accumulation of cyclic AMP was examined in intact mouse melanoma cells of varying metastatic potential. F 1 cells (low metastatic potential) had significantly greater cyclic AMP levels in response to all three hormones than F 5 (intermediate metastatic potential) and F 10 (high metastatic potential) cells. The ranking of the response was as follows: MSH, F 1 > F 5 > F 10 , ACTH, F 1 > F 5 > F 10 , PGE, F 1 > F 10 > F 5 . In contrast to the above, the degree of hormonal stimulation of adenylate cyclase in broken cell preparations was virtually identical in all three melanoma cell lines. Control enzyme activity was depressed in both F 5 and F 10 relative to F 1 . The conflicting results between studies of intact vs. broken cell preparations could not be explained by increased cyclic AMP phosphodiesterase activity in F 5 and F 10 cells. We conclude that as the melanoma cells increase in metastatic potential, there is a significant loss in the ability of their cyclic AMP system to respond appropriately to hormonal stimuli.