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Membrane bound and cellular cationic changes associated with insulin stimulation of cultured cells
Author(s) -
Sanui Hisashi,
Rubin A. Harry
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040960302
Subject(s) - insulin , intracellular , incubation , stimulation , metabolism , thymidine , efflux , embryo , in vitro , endocrinology , biology , medicine , dna synthesis , extracellular , carbohydrate metabolism , chemistry , biochemistry , microbiology and biotechnology
Insulin was employed as a stimulant in our continuing investigations of the molecular mechanisms involved in the coordinate control of cellular metabolism and growth. Incubation of chicken embryo fibroblasts for 16 hours in media containing 0–0.1 U insulin/ml resulted in a 17‐fold increase in the rate of 3 H‐thymidine incorporation into DNA. Concomitantly, there were graded increases in intracellular K + (14%) and Mg 2+ (22%) and no significant change in Ca 2+ . These changes in cation content occurred within 10 to 30 minutes and preceded the changes in 3 H‐thymidine incorporation. Insulin produced a consistent graded decrease in externally bound Mg 2+ and Ca 2+ and a concomitant increase in bound Na + and K + with no significant change in the rates of K + and Mg 2+ efflux. The results are consistent with the concept of Mg 2+ as a second messenger for insulin action, as well as with the more general hypothesis that Mg 2+ is the central agent in the coordinate control of metabolism and growth in animal cells.