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Effect of phosphate esters, nucleotides and nucleosides on 5′‐nucleotidase of cultured mouse macrophages
Author(s) -
Lazdins Janis,
Karnovsky Manfred L.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040960114
Subject(s) - 5' nucleotidase , adenosine , nucleotidase , adenosine monophosphate , extracellular , nucleotide , enzyme , chemistry , biochemistry , uridine , cell culture , enzyme assay , stimulation , microbiology and biotechnology , biology , endocrinology , rna , genetics , gene
Mouse peritoneal macrophages elicited by intraperitoneal injection of sodium caseinate exhibit low levels of ecto‐5′‐nucleotidase (E. C. 3.1.3.5) activity in contrast to macrophages obtained by peritoneal lavage. When elicited cells were cultured under standard conditions in the presence of serum, a 2.5‐fold increase in 5′‐nucleotidase activity was observed over a period of 48 hours. Addition of adenosine monophosphate to the culture medium led to an augmented (5‐fold) increase in the specific activity (per unit cell protein) as well as an absolute increase (per culture plate) of 5′‐nucleotidase. Other adenosine‐containing compounds also had stimulatory effects. The levels of this enzyme thus appear to be regulated by the extracellular levels of adenosine nucleotides. The product of the enzymatic reaction–adenosine–when added to the medium exhibited a toxic effect on these cells–as did adenosine monophosphate. However, the former substance did not augment the increase in enzyme activity during culture. The toxic effect could be suppressed when the cells were cultured in the presence of uridine 5′‐monophosphate. The latter substance also depressed the stimulation of enzyme activity due to AMP.