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Satellite DNA in differentiating chick tissues
Author(s) -
Ayres Kathleen Nelson
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040960113
Subject(s) - satellite dna , dna , satellite , biology , somatic cell , mitochondrial dna , microbiology and biotechnology , biophysics , biochemistry , genome , gene , physics , astronomy
Embryonic chick DNA from different tissues was examined for differences which might indicate specific DNA amplification in somatic cells. The problem was approached by determining the DNA compositional heterogeneity and searching for possible variation in different tissues of the 12‐day chick. Neural retina, muscle, and whole decapitated (general) chick DNA were analyzed in CsCl and Cs 2 SO 4 density gradients. While overloaded CsCl gradients showed a main band (ρ = 1.701 g/cm 3 ) and a heavy shoulder (ρ = 1.716 g/cm 3 ), overloaded Cs 2 SO 4 gradients displayed a main band (ρ = 1.426 g/cm 3 ) and a discrete heavy satellite (ρ = 1.447 g/cm 3 ) This satellite, comprising approximately 1% of the whole cell DNA, appeared to be of nuclear origin and not related to mitochondrial DNA, which was found to have a density of 1.426 g/cm 3 in Cs 2 SO 4 . No differences were found in the densities of the main band or the satellite DNA in the DNA samples isolated from the different tissues. However, the method of DNA isolation was found to be of crucial importance when comparing satellite DNA's among different tissues.