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Erythroid stem cells in friend‐virus infected mice
Author(s) -
Opitz Uta,
Seidel HansJoachim,
Bertoncello Ivan
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040960112
Subject(s) - bone marrow , spleen , biology , erythropoietin , in vitro , population , virus , erythropoiesis , incubation , colony forming unit , virology , immunology , andrology , microbiology and biotechnology , medicine , endocrinology , anemia , biochemistry , genetics , environmental health , bacteria
The erythropoietic stem cell compartment was studied in Friend‐virus (polycythemic strain, FV‐P) infected DBA/2 and NMRI mice with the CFU E and BFU E technique. Early after infection there was a depression in CFU E number in bone marrow and spleen, followed by an increase of the CFU E concentration, earlier and more pronounced in the spleen than in the marrow. Three days after FV‐P infection an erythropoietin (Ep) independent CFU E population started to grow and replaced the normal Ep‐dependent population within 8 to 12 days. The shift to Ep independency was not gradual. CFU E colonies of FV‐P infected bone marrow cells were two to three times larger than control colonies after three days in vitro incubation. BFU E colonies increased in number during the first days of infection, but were totally lost after more than ten days. After velocity sedimentation of bone marrow cells of FV‐P infected animals, however, the BFU E containing fractions showed normal BFU E colony growth and normal Ep sensitivity. In unfractionated bone marrow cell cultures BFU E colony growth could be observed later than ten days post infection when the cultures were refed with medium. It was therefore concluded that the loss of BFU E colony growth after FV‐P infection was an in vitro artefact due to inadequate culture conditions.