Premium
Spontaneous mutation rate to thioguanine resistance is decreased in polyploid hamster cells
Author(s) -
Morrow John,
Stocco Douglas,
Barron Emily
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040960110
Subject(s) - ploidy , polyploid , chinese hamster , biology , hamster , mutation , genetics , colchicine , microbiology and biotechnology , chromosome , phosphoglycerate kinase , mutation rate , cell culture , gene
The mutation rate to thioguanine resistance was 3.11 × 10 −6 in a near diploid V79 hamster cell line and 7.58 × 10 −8 in a near tetraploid derivative produced with colchicine. The specific activities of glucose‐6‐phosphate dehydrogenase and phosphoglycerate kinase of the tetraploid line were greater than that of the diploid which suggests that twice the number of active X chromosomes were present in the tetraploid. These results are compatible with the hypothesis that spontaneous variants resistant to thioguanine arise through mutation and chromosomal segregation, as has been suggested for induced mutations in tetraploid hamster cells.