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Isolation of high‐rate DNA synthetic cells by Con A chromatography
Author(s) -
Wallenbrock Mark A.,
Killion Jerald J.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040950204
Subject(s) - dna , thymidine , affinity chromatography , dna synthesis , population , cell , lectin , chromatography , chemistry , microbiology and biotechnology , column chromatography , biochemistry , biology , demography , sociology , enzyme
The separation of L1210 cells with columns of Con A‐derivatized nylon was investigated. Most of the cells bound to the column irreversibly. The binding was lectin‐specific. Cells were pulse labeled with 3 H‐thymidine and applied to Con A columns. Those cells not binding the columns were enriched in incorporated thymidine compared to the unseparated population. Data is presented which suggests that a small, synchronized fraction of cells synthesizing DNA at a high rate is reduced in Con A‐nylon affinity. It is proposed that L1210 cell DNA synthesis is not uniform in rate and that changes in this rate are related to changes in the ability of cells to bind Con A‐nylon.