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In vitro and in vivo actions of zinc ion affecting cellular substances which influence host metabolic responses to inflammation
Author(s) -
Mapes Carol A.,
Bailey Paul T.,
Matson Charles F.,
Hauer Edward C.,
Sobocinski Philip Z.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040950114
Subject(s) - in vivo , in vitro , mediator , inflammation , exudate , arachidonic acid , metabolism , chemistry , antagonism , zinc , endogeny , biochemistry , lipopolysaccharide , long term potentiation , pharmacology , biology , endocrinology , immunology , enzyme , receptor , botany , microbiology and biotechnology , organic chemistry
Glycogen‐stimulated rabbit peritoneal exudate cells (polymorphonuclear leukocytes, PMN) produce prostaglandins (PG) and substances which induce alterations (mediators) in experimental animals characteristics of host metabolic responses to infection and other acute inflammatory stresses. The effect of Zn 2+ on mediator production and PG synthesis was examined because: Zn homeostasis is perturbed during infection, Zn is known to regulate some cellular functions, and there appears to be an interrelationship between PG synthesis and mediator production Using exudate cells, 2 mM Zn 2+ causec complete inhibition of in vitro PG synthesis as assessed by conversion of [1− 14 C] arachidonic acid into PG. This concentration of Zn 2+ also inhibited production of substances mediating plasma Zn depression, hepatic amino acid "uptake," fever, and neutrophil release from bone marrow. Conversely, Zn 2+ did not inhibit in vivo metabolic responses to these mediators. Zn‐pretreatment of rabbits or simultaneous injection of Zn 2+ and crude PMN‐derived pyrogenic activity resulted in prolongation of fever. It is suggested that this action of Zn 2+ may be attributed to either stabilization of cyclic AMP through inhibition of phosphodiesterase or a Zn‐mediator interaction which stabilizes crude endogenous pyrogen The potential physiological significance of these results includes: possible potentiation of the host's defense mechanisms by Zn 2+ and its utilization for prolongation of fever to determine its effect on potentially temperature‐dependent host defense mechanisms.