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The stimulation of DNA synthesis by cytochalasin B in proliferative epidermal and dermal cells
Author(s) -
Rothberg Simon,
Nancarrow Grace E.,
Church Virgene L.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040950109
Subject(s) - cytochalasin b , microfilament , dna synthesis , cytochalasin , stimulation , epidermis (zoology) , biology , microbiology and biotechnology , cell cycle , thymidine , cell , cytochalasin d , mitosis , dna , cytoskeleton , endocrinology , biochemistry , anatomy
Cytochalasin B influences a variety of cellular events that are associated with the contractile microfilament system and the formation of binucleate cells. Along with the formation of binucleate cells, cytochalasin B also causes an acceleration of cells from G 1 to S in the cell cycle By pulsing with cytochalasin B for 30 minutes and allowing for a previously established lag time (17.5 hours) a stimulation of thymidine incorporation into DNA of proliferative epidermal and dermal cells was found in both control and stripped epidermis Autoradiographic analysis confirmed that the stimulation was due to an increased number of basal cells accelerated from G 1 to S phase. A minimal number of binucleate basal cells, 1 in 300, was observed, which suggests that the stimulated synthesis is independent of binucleate cell formation. The amount of stimulation is maximum with cytochalasin B concentration pulse between 5γ and 30γ/ml The results suggest a possible link in coupling cell membrane and surface events with subsequent increased cell nuclei synthetic activity.