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Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblasts
Author(s) -
Ronald Zielke H.,
Ozand Pinar T.,
Tyson Tildon J.,
Sevdalian David A.,
Cornblath Marvin
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040950106
Subject(s) - glutamine , ploidy , wi 38 , biochemistry , biology , glucose uptake , chemistry , endocrinology , amino acid , insulin , gene
Human diploid fibroblasts utilize both glucose and glutamine as energy sources. The utilization of glutamine by fibroblasts is regulated by glucose, and vice versa. This conclusion is supported by the following observations: (1) essentially identical growth rates were observed in Eagle's minimum essential medium (MEM) 3 in which the glucose concentration was either 5.5 mM or was maintained between 25 and 40 μM, (2) the total glutamine utilization by fibroblasts increased at least 30% in medium with 25 μM to 70 μM glucose compared to medium with 5.5 mM glucose, while the rate of glutamine‐1 or 5‐ 14 C oxidation to CO 2 increased 5‐fold as the glucose concentration was decreased to zero, (3) 2 mM glutamine inhibited glucose‐6‐ 14 C oxidation by 88% and stimulated glucose‐1‐ 14 C by 77% in log phase cells and (4) glutamine oxidation in normal medium contributed approximately 30% of the energy requirement of human diploid fibroblasts.