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Density‐dependent effects of oxygen on the growth of mammalian fibroblasts in culture
Author(s) -
Taylor William G.,
Camalier Richard F.,
Sanford Katherine K.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040950105
Subject(s) - laboratory flask , cell culture , oxygen , cell growth , lactic acid , glycolysis , plating efficiency , biology , microbiology and biotechnology , inoculation , growth rate , growth medium , yield (engineering) , fibroblast , metabolism , cell , biochemistry , chemistry , in vitro , bacteria , immunology , materials science , genetics , geometry , mathematics , organic chemistry , metallurgy
Various concentrations of oxygen were used to determine the optimum culture medium P O 2for survival and proliferation of attached human and mouse fibroblasts grown from different inoculum sizes. When T−15 flasks were seeded with ⪕ 2 × 10 4 cells (⪕ 1.3 × 10 3 cells/cm 2 ), the highest plating efficiencies and cell yields were obtained with a culture medium P O 2of 40—60 mm Hg. At higher inoculum sizes (10 5 cells per T‐15) used routinely for mass cultures, no difference in cell yield or glycolytic activity was observed between cultures gassed with atmospheric, i.e., 18% O 2 (growth medium P O 2≅ 125—135 mm Hg) and those gassed with 1% O 2 (growth medium P O 2≅ 40—60 mm Hg). The enhanced clonal growth observed at the latter P O 2results from an increased proliferation rate rather than more efficient attachment and survival of inoculated cells. Glucose uptake and lactic acid accumulation were increased in sparse cultures sparged with 1% O 2 . A slight extension of lifespan was observed in WI−38 cells serially subcultured with a gas phase of 1% O 2 .