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Changes in membrane dynamics associated with myogenic cell fusion
Author(s) -
Herman B. A.,
Fernandez S. M.
Publication year - 1978
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040940303
Subject(s) - dynamics (music) , microbiology and biotechnology , fusion , cell fusion , lipid bilayer fusion , cell , membrane , biology , chemistry , physics , genetics , linguistics , philosophy , acoustics
Changes in membrane dynamic properties associated with membrane fusion are studied employing in vitro myoblast fusion as a model system. We utilize a microscopic fluorescence relaxation approach which makes feasible the study of local variations in membrane dynamics within surface subdomains of single intact cells. Studies of the average rotational mobility of the fluorescent probe‐1‐anilino‐naphthalene‐8‐sulfonate by this technique indicate that myoblast fusion activity is preceded by a generalized increased in membrane fluidity and that areas of cell contact between fusing cells exhibit higher fluidity and polarity, locally, than non‐fusion regions.